Formation - Optical Control of Brain Functioning with Optogenetics and Wave Front Shaping

Scientific and technical environment of the training course

Institut de la vision

- UMR 7210

Institut Carnot Voir et Entendre

COURSE DIRECTORS

Valentina EMILIANI

Senior researcher

UMR 7210

Eirini PAPAGIAKOUMOU

Researcher

UMR 7210

LOCATION

PARIS (75)

ORGANISATION

8 days
From 09:00 am to 06:00 pm
Training course in English
From 7 to 20 attendees

The next session will take place in March 2024. Registration will open in July 2023.

PEDAGOGICAL METHODS

Lectures (23 h), workshops (28 h) and presentations of projects (8 h)
Files in PDF format will be made available to the trainee.

TRAINING FEES

3000 Euros

AT THE END OF THE TRAINING COURSE

- Satisfaction survey from trainees
- A training certificate is delivered

COURSE DATE

Nous consulter

2025

January	February	March 25180	April
May	June	July	August
Sept	Oct	Nov	Déc

OBJECTIVES

Learn how to choose the most suitable combination of actuator, illumination methods and targeting strategy for each specific application

Acquire the skills and technologies required to design and build up an optical system for optical control of neuronal circuits

AUDIENCE

Neurophysiologists, physicists (researchers, PhD students or engineers)

PRE-REQUIREMENT

Attendees should have a strong background in neurosciences or in cell biology or in optical microscopy. To adapt the programme to trainees' expectations, we invite the attendees to download and fill out the survey HERE and submit it when performing the pre-registration.

TRAINING PROGRAMME

Lectures
- Introduction to optogenetics
- Wave front shaping and liquid crystal matrix
- Viral vector design
- Computer generated holography
- Generalized phase contrast
- Temporal focusing
- Three-dimensional light patterning and temporal focusing
- Two-photon optogenetics: scanning, spiral scanning, parallel illumination: examples and comparison among the different approaches
- All-optical interrogation of brain circuits
- Voltage and calcium imaging
- Two-photon all-optical circuits manipulation in freely moving mice
- Patterned voltage and calcium imaging
- Three photon imaging and optogenetics

Practical courses (in small rotating groups of 7 attendees max with 2 trainers per group)
- How to build up a holographic optical set-up (optical design and software)
- In vitro and in vivo all-optical manipulation of neuronal circuits
- Two-photon all-optical circuits manipulaiotn in freely moving mice
- Two-photon scanless voltage imaging
- Projects from applicants (practical): proposition of projects by the attendees and discussion on their feasibility

SPEAKERS

V. Emiliani, E. Papagiakoumou, N. Accanto, E. Ronzitti, D. Dalkara, D. Tanese, C. Grimm, R. Sims (researchers, IDV, Paris), C. Wyart (researcher, ICM, Paris), D. Oron (professor, Weizmann Institute), M. Lin (researcher, Stanford University), J. Vierock (researcher, Charité - Universitätsmedizin Berlin) and C. Xu (professor, Cornell University).

Avis des stagiaires

"This course exceed my expections in terms of training quality, quality of speakers / content, and the opportunity to meet coll new scientits ! I would recommend having some reserved time for whole group questions / discussion" Rosana M., Université

"Perfect course, great coordination and organization, extremely helpful lab members" Sakir Kaan C., Centre de recherche

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